FISH banding methods: applications in research and diagnostics.

Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish

The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two ...

Molecular cytogenetics: an indispensable tool for cancer diagnosis.

Cytogenetic aberrations may escape detection or recognition in traditional karyotyping. The past decade has seen an explosion of methodological advances in molecular cytogenetics technology. These cytogenetics techniques add color to the black and white world of conventional banding. Fluorescence in-situ hybridization (FISH) study has emerged as an indispensable tool for both basic and clinical...

Insulin-like growth factor I gene polymorphism associated with growth traits in beluga (Huso huso) fish

The aim of the present study was to detect polymorphism in Insulin like growth factor-I (IGF-I) gene of beluga (Huso huso) fish using PCR-SSCP technique and also investigation of its association with growth traits (condition factor, body length and weight). A total of 150 specimens of beluga were randomly selected and DNA was isolated from caudal fin using modified salting out method. Then two ...

A Rare Case of Klinefelter Syndrome Patient with Quintuple Mosaic Karyotype, Diagnosed by GTG-Banding and FISH

Comparison of classical cytogenetics versus interphase FISH in diagnosis of mosaic form of Turner syndrome

 Abstract  Background: Mosaic form of turner syndrome that represented by two or more  cell lines in an affected individual, often has limitation for detection with classical  cytogenetic methods. The present study was carried out to compare the efficiency of  interphase Fluorescence In Situ Hybridisation (FISH) and cytogenetic techniques in  detection of mosaic form of turner syndrome.  Method...